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Immunoluminometric assay of chromogranin A in serum with commercially available reagents.
- Source :
-
Clinical chemistry [Clin Chem] 1992 Nov; Vol. 38 (11), pp. 2267-72. - Publication Year :
- 1992
-
Abstract
- Chromogranin A (Cg A) is a useful marker of neuroendocrine neoplasia in humans. Here we describe an immunoluminometric assay (ILMA) for measuring Cg A in serum, with use of a new tube as a solid phase. The new tube has a large surface area and is coated with a polyclonal antibody. Optimized coating conditions provide a high IgG adsorption to the polystyrene wall. Serum is added to the coated tube and incubated for 2 h or overnight. After washing the tubes, acridinium ester-labeled monoclonal antibody against Cg A is added and incubation is continued for 2 h. The tubes are washed again and the bound luminescence is measured. The assay is very sensitive (detection limit 1 microgram/L, if 25 microL of serum is used), is specific for human Cg A, and offers a wide dynamic range (1-1000 micrograms/L). The range in healthy humans is 10-53 micrograms/L (median 30 micrograms/L). The correlation of serum Cg A to creatinine and parathyroid hormone is described. We also report the influence of intravenous calcium injection on Cg A concentrations in serum.
- Subjects :
- Antibodies, Monoclonal
Catecholamines blood
Chromogranin A
Creatinine blood
Drug Stability
Humans
Immunoassay statistics & numerical data
Kinetics
Parathyroid Hormone blood
Radioimmunoassay
Reference Values
Chromogranins blood
Immunoassay methods
Luminescent Measurements
Reagent Kits, Diagnostic
Subjects
Details
- Language :
- English
- ISSN :
- 0009-9147
- Volume :
- 38
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Clinical chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 1424122