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Developmental patterns of PDGF B-chain, PDGF-receptor, and alpha-actin expression in human glomerulogenesis.
- Source :
-
Kidney international [Kidney Int] 1992 Aug; Vol. 42 (2), pp. 390-9. - Publication Year :
- 1992
-
Abstract
- Expression of PDGF B-chain and the PDGF receptor beta-subunit (PDGFR beta) is detected immunocytochemically during the development of glomeruli in human kidneys of 54 to 105 days gestational age. During the early stages (vesicular, comma-shape and S-shape) of glomerulogenesis, PDGF B-chain is localized to differentiating epithelium of the glomerular vesicle, while PDGFR beta is expressed in the undifferentiated metanephric blastema, vascular structures, and interstitial cells. During this stage PDGF may be acting as a paracrine growth factor and as a chemoattractant acting to recruit mesangial progenitor cells into the developing glomerulus. As the glomerular tuft forms, both PDGF B-chain and PDGFR beta can be detected in an arboreal pattern radiating from the hilus of the glomerular tuft. Immunocytochemical studies using markers specific to endothelium (Ulex europaeus I lectin, Factor VIII related antigen), and smooth muscle (alpha-smooth muscle actin), indicate that the PDGF B-chain and PDGFR beta are both expressed primarily by mesangial cells. During this stage, PDGF may be acting primarily to provide an autocrine factor to mediate further mesangial cell proliferation. Glomerular expression of alpha-smooth muscle actin is limited to later stages of glomerulogenesis; at these stages the pattern of expression is similar to that of PDGF-B chain and PDGFR beta. The upregulation of mesangial PDGF, PDGFR beta, and alpha-smooth muscle actin expression that has been identified in some disease states in both humans and experimental animals appears to represent a recapitulation of this normal developmental process.
- Subjects :
- Cell Division
Gestational Age
Glomerular Mesangium cytology
Glomerular Mesangium embryology
Glomerular Mesangium metabolism
Humans
Immunohistochemistry
Muscle, Smooth embryology
Muscle, Smooth metabolism
Up-Regulation
Actins metabolism
Kidney Glomerulus embryology
Kidney Glomerulus metabolism
Platelet-Derived Growth Factor metabolism
Receptors, Platelet-Derived Growth Factor metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0085-2538
- Volume :
- 42
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Kidney international
- Publication Type :
- Academic Journal
- Accession number :
- 1405322
- Full Text :
- https://doi.org/10.1038/ki.1992.300