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Light-induced degradation of D2 protein in isolated photosystem II reaction center complex.
- Source :
-
FEBS letters [FEBS Lett] 1992 Oct 12; Vol. 311 (1), pp. 33-6. - Publication Year :
- 1992
-
Abstract
- When isolated photosystem II reaction centers from spinach are exposed to photoinhibitory light in the presence of an electron acceptor, breakdown products of the D2 protein at 28, 25, 23, 18, 9, 5 and 4.5 kDa are detected by immunoblotting with a monospecific anti-D2 polyclonal antibody. In a time-course experiment the 23 and 4.5 kDa fragments show a transient appearance, whilst the others are photoaccumulated. The regions of the D2 protein containing the cleavage sites for the 28 and 18 kDa photoinduced fragments have been identified. Significant degradation of D2 takes place only in the presence of an electron acceptor, and breakdown of the protein is partially prevented by serine-type protease inhibitors.
- Subjects :
- Amino Acid Sequence
Blotting, Western
Endopeptidases drug effects
Endopeptidases metabolism
Molecular Sequence Data
Peptide Fragments isolation & purification
Photic Stimulation
Photosynthetic Reaction Center Complex Proteins isolation & purification
Photosystem II Protein Complex
Serine Proteinase Inhibitors pharmacology
Peptide Fragments metabolism
Photosynthetic Reaction Center Complex Proteins metabolism
Plants metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0014-5793
- Volume :
- 311
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- FEBS letters
- Publication Type :
- Academic Journal
- Accession number :
- 1397286
- Full Text :
- https://doi.org/10.1016/0014-5793(92)81360-x