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Detection of VAC-beta (annexin-8) in human placenta.
- Source :
-
FEBS letters [FEBS Lett] 1992 Jul 13; Vol. 306 (1), pp. 85-9. - Publication Year :
- 1992
-
Abstract
- A 36 kDa calcium/phospholipid binding protein in human placenta was identified as VAC-beta (annexin-8) by a combination of immunological and peptide mapping analyses. The protein is a minor product in placenta, accounting for less than 1% of extracted annexins. From 150 g of tissue, only 100 micrograms of the protein was isolated. By anion-exchange chromatography on diethylaminoethyl-cellulose annexin-8 coeluted with annexin-3. By gel filtration, the protein chromatographed as a broad peak, where half the product eluted as a monomer and half eluted as a heterodimer that was associated with a 10 kDa subunit. The combination of annexin-8 being a minor component in standard annexin preparations and it co-eluting with annexin-3 by ion exchange chromatography are likely to account for the failure of other labs to characterize the product.
Details
- Language :
- English
- ISSN :
- 0014-5793
- Volume :
- 306
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- FEBS letters
- Publication Type :
- Academic Journal
- Accession number :
- 1385789
- Full Text :
- https://doi.org/10.1016/0014-5793(92)80843-6