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Interleukin 4 and tumour necrosis factor alpha induce different adhesion pathways in endothelial cells for the binding of peripheral blood lymphocytes.
- Source :
-
Scandinavian journal of immunology [Scand J Immunol] 1992 Oct; Vol. 36 (4), pp. 575-85. - Publication Year :
- 1992
-
Abstract
- We demonstrated that tumour necrosis factor alpha (TNF-alpha) and interleukin 4 (IL-4) increased endothelial cell (EC) adhesiveness for peripheral blood lymphocytes (PBL) by promoting transcription and protein synthesis. The different kinetics observed with TNF-alpha and IL-4 suggest the involvement of different adhesion molecules. Blocking adhesion assays and immunofluorescence analysis showed that PBL adhesion to endothelial cells involves different pair adhesion molecules. Whereas IL-4 promoted an LFA-1-dependent/ICAM-1-independent adhesion pathway on EC, TNF-alpha stimulated an LFA-1-dependent/ICAM-1-dependent adhesion pathway on EC. In contrast, VLA-4/VCAM-1 molecules were involved in PBL adhesion both to IL-4 and to TNF-alpha-stimulated EC. Finally, we found that a CD2-dependent/LFA-3-independent adhesion pathway was mainly involved in IL-4-stimulated EC.
- Subjects :
- Antigens, CD physiology
Antigens, Differentiation, T-Lymphocyte physiology
CD2 Antigens
CD58 Antigens
Cell Adhesion drug effects
Cell Adhesion Molecules physiology
Cells, Cultured
Cycloheximide pharmacology
Dactinomycin pharmacology
Endothelium, Vascular drug effects
Humans
Lymphocyte Function-Associated Antigen-1 physiology
Lymphocytes drug effects
Membrane Glycoproteins physiology
Receptors, Immunologic physiology
Receptors, Very Late Antigen physiology
Vascular Cell Adhesion Molecule-1
Endothelium, Vascular physiology
Interleukin-4 pharmacology
Lymphocytes physiology
Tumor Necrosis Factor-alpha pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0300-9475
- Volume :
- 36
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Scandinavian journal of immunology
- Publication Type :
- Academic Journal
- Accession number :
- 1384114
- Full Text :
- https://doi.org/10.1111/j.1365-3083.1992.tb03226.x