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The rat angiotensin II AT1A receptor couples with three different signal transduction pathways.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 1992 Jul 31; Vol. 186 (2), pp. 1094-101. - Publication Year :
- 1992
-
Abstract
- To examine whether the subpopulation of the rat type 1 angiotensin II (AII) receptor (AT1A) couples with a single or multiple signal transduction pathways, we constructed Chinese hamster ovary (CHO) cell lines producing the recombinant receptor. The expressed AT1A receptor exhibits typical pharmacological characteristics of the AT1 receptor, known to mediate the main physiological function of AII. Addition of AII to the CHO cells induced a rapid, transient increase in intracellular free Ca2+ concentrations ([Ca2+]i) followed by a lower, sustained phase. Nicardipine, a blocker of voltage-dependent L-type Ca2+ channels, attenuated the transient [Ca2+]i response and abolished the sustained phase. The transient phase was also reduced dose-dependently by the phospholipase C inhibitor neomycin. Furthermore, AII inhibited forskolin-evoked cAMP accumulation. These data suggest, although another subpopulation named AT1B is present, that the rat AT1A receptor can independently couple with all three signal transduction pathways known to be induced by AII: i.e., i) activation of phospholipase C resulting in InsP3 generation with a subsequent release of intracellularly stored Ca2+, ii) activation of dihydropyridine-sensitive voltage-dependent Ca2+ channels, and iii) inhibition of adenylate cyclase activity.
- Subjects :
- 3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester pharmacology
Angiotensin II metabolism
Animals
Binding, Competitive
CHO Cells
Cricetinae
Egtazic Acid pharmacology
Kinetics
Neomycin pharmacology
Nicardipine pharmacology
Rats
Receptors, Angiotensin drug effects
Receptors, Angiotensin genetics
Recombinant Proteins drug effects
Recombinant Proteins metabolism
Transfection
Type C Phospholipases metabolism
Angiotensin II pharmacology
Calcium metabolism
Colforsin pharmacology
Cyclic AMP metabolism
Receptors, Angiotensin physiology
Signal Transduction drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 0006-291X
- Volume :
- 186
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 1379799
- Full Text :
- https://doi.org/10.1016/0006-291x(92)90859-j