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Transient increase in renal insulin-like growth factor binding proteins during initial kidney hypertrophy in experimental diabetes in rats.
- Source :
-
Diabetologia [Diabetologia] 1992 Jun; Vol. 35 (6), pp. 589-93. - Publication Year :
- 1992
-
Abstract
- The insulin-like growth factors, insulin-like growth factor I and insulin-like growth factor II are bound to six distinct classes of insulin-like growth factor binding proteins (IGFBPs) in the circulation and in extracellular fluids. Diabetic renal hypertrophy is preceded by a transient increase in kidney insulin-like growth factor I suggestive of a renotropic function for insulin-like growth factor I. In order to examine a possible involvement of IGFBPs in initial diabetic kidney growth and in kidney insulin-like growth factor I accumulation, we studied rat kidney IGFBPs by ligand blotting during the first 4 days after induction of diabetes. Six distinct bands were identified in kidney and liver tissue with apparent molecular weight values of 38-47 (doublet), 34, 30, 24 and 20 kDa. The 38-47 kDa doublet band probably corresponds to the insulin-like growth factor binding subunit of IGFBP-3, the 24 kDa band to IGFBP-4 and the 30 kDa band to IGFBP-1 and/or IGFBP-2, as these IGFBPs in rats have similar molecular weight. In untreated diabetic rats a transient increase in the kidney 30 kDa band was demonstrable 24 h after induction of diabetes with a maximal rise (two-fold) after 48 h, followed by a decrease to baseline values after 4 days. In untreated diabetic rats the 38-47 kDa doublet band also increased (two-fold) in kidney during the first 2 days after induction of diabetes, followed by a subsequent decrease. Insulin-treatment prevented both the increase in the 30 kDa and in the 38-47 kDa bands.(ABSTRACT TRUNCATED AT 250 WORDS)
- Subjects :
- Animals
Blood Glucose metabolism
Carrier Proteins isolation & purification
Diabetes Mellitus, Experimental drug therapy
Diabetes Mellitus, Experimental pathology
Electrophoresis, Polyacrylamide Gel
Hypertrophy
Insulin therapeutic use
Insulin-Like Growth Factor Binding Protein 2
Insulin-Like Growth Factor Binding Protein 4
Insulin-Like Growth Factor Binding Proteins
Kidney drug effects
Kidney pathology
Liver metabolism
Male
Molecular Weight
Organ Size drug effects
Rats
Rats, Inbred Strains
Carrier Proteins metabolism
Diabetes Mellitus, Experimental metabolism
Insulin-Like Growth Factor I metabolism
Kidney metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0012-186X
- Volume :
- 35
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Diabetologia
- Publication Type :
- Academic Journal
- Accession number :
- 1377139
- Full Text :
- https://doi.org/10.1007/BF00400489