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Interstrain cross-reactive idiotypes on monoclonal antibodies to an encephalitogenic myelin basic protein peptide.
- Source :
-
Clinical immunology and immunopathology [Clin Immunol Immunopathol] 1992 Apr; Vol. 63 (1), pp. 74-83. - Publication Year :
- 1992
-
Abstract
- In order to assess the role of idiotype (Id) and the anti-Id network in murine experimental autoimmune encephalomyelitis (EAE), Id-bearing monoclonal antibodies (mAb) to human myelin basic protein (MBP) peptide acetyl 1-9, as well as mAb anti-Id, were developed in EAE-susceptible PL/J mice (H-2u). These mice recognize MBP residues acetyl 1-9 as an encephalitogenic determinant. Reactivities of PL/J Id-bearing mAbs to MBP and to MBP peptides were identical to those of mAbs generated against the same MBP peptide in EAE-resistant BALB/c mice (H-2d), even though isotypes of the mAbs differed. By using an inhibitory ELISA and immunoblotting, it was demonstrated that one PL/J mAb anti-Id recognized a public or framework Id, whereas another PL/J mAb-anti Id was directed to a private Id more restricted to the paratopic site. Two Id-bearing PL/J mAbs shared a cross-reactive Id (IdX) on the light chain, and an interstrain IdX was present on both the heavy and light chains of mAbs raised in PL/J and BALB/c mice to the same MBP peptide. The PL/J mAb anti-Id was capable of cross-regulating the production of Id-bearing mAbs by hybridomas across murine strains. These findings suggest that a restrictive family of germ-line genes encode for these Id-bearing antibodies to MBP peptide, irrespective of whether the MBP peptide is encephalitogenic in the murine strain immunized. Manipulation of the Id network may provide a means for modifying autoimmune demyelinating diseases of the central nervous system.
- Subjects :
- Animals
Binding, Competitive
Cross Reactions
Disease Models, Animal
Enzyme-Linked Immunosorbent Assay
Female
Flow Cytometry
Gene Expression Regulation
Hybridomas metabolism
Immunoblotting
Immunoglobulin Isotypes
Immunoglobulin M biosynthesis
Mice
Mice, Inbred Strains
Antibodies, Monoclonal immunology
Encephalomyelitis, Autoimmune, Experimental immunology
Immunoglobulin Idiotypes immunology
Myelin Basic Protein immunology
Peptide Fragments
Subjects
Details
- Language :
- English
- ISSN :
- 0090-1229
- Volume :
- 63
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Clinical immunology and immunopathology
- Publication Type :
- Academic Journal
- Accession number :
- 1375543
- Full Text :
- https://doi.org/10.1016/0090-1229(92)90096-7