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c-myc expression is down-regulated by cell-cell and cell-extracellular matrix contacts in normal hepatocytes, but not in hepatoma cells.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 1992 Apr 30; Vol. 184 (2), pp. 825-31. - Publication Year :
- 1992
-
Abstract
- Primary culture of adult rat hepatocytes resulted in marked increase of c-myc expression within a few hours. The high level of c-myc mRNA was maintained throughout culture on collagen-coated dishes, but decreased greatly with time during culture on collagen-gel or matrigel. Expression of c-myc was also down-regulated at high cell density. The decrease in its expression appeared closely related to inhibitions of DNA synthesis and cell spreading. In contrast, hepatoma H4TG cells showed a high level of c-myc expression which was not affected by culture on any extracellular matrices examined or by the cell density. These results suggest that up-regulation of expression of the c-myc gene is linked to G0 to G1 transition during cell cycle progression, which in normal hepatocytes is strictly regulated by cell-cell and cell-extracellular matrix interactions, but that this control mechanism is defective in malignant hepatic tumor cells.
- Subjects :
- Animals
Cell Line
Cells, Cultured
Kinetics
Male
RNA genetics
RNA isolation & purification
Rats
Rats, Inbred Strains
Cell Communication
DNA Replication
Extracellular Matrix physiology
Gene Expression Regulation
Gene Expression Regulation, Neoplastic
Genes, myc
Liver physiology
Liver Neoplasms, Experimental genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0006-291X
- Volume :
- 184
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 1374242
- Full Text :
- https://doi.org/10.1016/0006-291x(92)90664-7