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A new method for the encapsulation of mammalian cells.
- Source :
-
Cytotechnology [Cytotechnology] 1991 Oct; Vol. 7 (2), pp. 121-30. - Publication Year :
- 1991
-
Abstract
- A new encapsulation method was developed for the cultivation of mammalian cells. The capsules were produced using a solution of sodium cellulose sulphate (CS)(1.5%) and poly-dimethyl-diallyl-ammonium chloride (PDMDAAC). When CS droplets fell into the precipitation bath consisting of a 2% solution of PDMDAAC, immediately a membrane at the interphase was built up. The influences of varying encapsulation process parameters on capsule characteristics, cell growth, and monoclonal antibody production were tested. This new method showed advantages when compared to other methods mainly due to time simplicity of the whole process.
- Subjects :
- Animals
Antibodies, Monoclonal analysis
Antibodies, Monoclonal biosynthesis
Capsules
Cell Line
Cellulose analogs & derivatives
Culture Techniques methods
Hybridomas immunology
Immunoglobulin G analysis
Immunoglobulin G biosynthesis
Mice
Polymers
Hybridomas physiology
Polyethylenes
Quaternary Ammonium Compounds
Subjects
Details
- Language :
- English
- ISSN :
- 0920-9069
- Volume :
- 7
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Cytotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 1367907
- Full Text :
- https://doi.org/10.1007/BF00350918