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Efficient and reproducible new semimicromethod for the detection and titration of HIV in human plasma.
- Source :
-
Journal of medical virology [J Med Virol] 1992 Nov; Vol. 38 (3), pp. 207-13. - Publication Year :
- 1992
-
Abstract
- A semimicromethod was established for isolating human immunodeficiency virus (HIV) in plasma using 48-well plates and a pool of peripheral blood mononuclear cells (PBMC) from several donors as targets for infection, which increases the efficiency of isolation by reducing the effect of variability due to diverse donor cell susceptibility to HIV infection. The addition of H9 cells to the PBMC cultures did not affect measurable titers. Nevertheless, it potentiated strongly virus replication in terms of p24 production in the supernatant of the wells with HIV isolates, thus facilitating interpretation of the results. The titration of a virus strain of a known titre and reverse transcriptase activity in parallel provided a constant parameter of efficiency and reproducibility within each experiment, permitting comparison with results from other laboratories. The reproducibility of the method was highly significant (r = 0.97, P < 0.001); 68% of the 22 plasma samples from HIV-infected individuals tested by this method were positive. The presence of plasma HIV titer correlated well (P < 0.02) with the low count of CD4+ cells of less than 300/mm3, but not with the presence of the p24 antigen in the serum.
- Subjects :
- Acquired Immunodeficiency Syndrome immunology
Acquired Immunodeficiency Syndrome microbiology
CD4-Positive T-Lymphocytes
Cell Count
Cell Line
Cells, Cultured
Female
HIV Core Protein p24 blood
HIV Infections immunology
HIV Seropositivity immunology
HIV Seropositivity microbiology
HIV-1 physiology
Humans
Leukocyte Count
Male
Reproducibility of Results
Time Factors
Virus Replication
HIV Infections microbiology
HIV-1 isolation & purification
Leukocytes, Mononuclear microbiology
Viremia microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 0146-6615
- Volume :
- 38
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Journal of medical virology
- Publication Type :
- Academic Journal
- Accession number :
- 1363119
- Full Text :
- https://doi.org/10.1002/jmv.1890380310