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Identification of 57 conventional RFLP and 6 VNTR systems with 32 DNA clones on chromosome 11p15.

Authors :
Takita K
Tanigami A
Tokino T
Jones C
Nakamura Y
Source :
Genomics [Genomics] 1992 Aug; Vol. 13 (4), pp. 1296-9.
Publication Year :
1992

Abstract

Fifty-four clones containing human inserts were selected from a cosmid library constructed from a somatic cell hybrid containing chromosome 11p15.3-p15.5 as its only human complement. In 32 of these clones, 63 polymorphic systems were identified with a panel of restriction enzymes: 57 conventional RFLP systems and 6 highly polymorphic VNTR systems. Although we examined the cosmid with only seven enzymes, 18 clones (including 6 VNTRs) were polymorphic with three or more enzymes. The results suggested that DNA sequences on the peritelomeric region of chromosome 11p tend to be highly variable. Because these markers are highly informative, they will be excellent resources for investigations of hereditary diseases and tumor suppressor genes in this region of chromosome 11.

Details

Language :
English
ISSN :
0888-7543
Volume :
13
Issue :
4
Database :
MEDLINE
Journal :
Genomics
Publication Type :
Academic Journal
Accession number :
1354646
Full Text :
https://doi.org/10.1016/0888-7543(92)90049-x