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A high-resolution cytogenetic map of 168 cosmid DNA markers for human chromosome 11.

Authors :
Hori T
Takahashi E
Tanigami A
Tokino T
Nakamura Y
Source :
Genomics [Genomics] 1992 May; Vol. 13 (1), pp. 129-33.
Publication Year :
1992

Abstract

We have constructed a high-resolution cytogenetic map with 168 DNA markers, including 90 RFLP markers for human chromosome 11. The cosmid clones were mapped by fluorescence in situ suppression hybridization, in which discrete fluorescent signals can be detected directly on prometaphase R-banded chromosomes. Although these cosmid clones were distributed throughout the chromosome, they had some tendency to localize in the regions of R-positive band, such as 11p15, 11p11.2, 11q13, 11q23, and 11q25. Since these regions of chromosome 11 are considered to contain genes responsible for certain genetic diseases, cancer breakpoints involved in chromosome rearrangements, and tumor-suppressor genes, this high-resolution cytogenetic map will contribute to the molecular characterization of such genes. This map will also provide many landmarks essential for construction of the complete physical map with contigs of cosmid and YAC clones.

Details

Language :
English
ISSN :
0888-7543
Volume :
13
Issue :
1
Database :
MEDLINE
Journal :
Genomics
Publication Type :
Academic Journal
Accession number :
1349569
Full Text :
https://doi.org/10.1016/0888-7543(92)90212-b