Back to Search
Start Over
Fluoride and phosphatidylserine induced inhibition of cytosolic insulin-degrading activity.
- Source :
-
Acta physiologica, pharmacologica et therapeutica latinoamericana : organo de la Asociacion Latinoamericana de Ciencias Fisiologicas y [de] la Asociacion Latinoamericana de Farmacologia [Acta Physiol Pharmacol Ther Latinoam] 1992; Vol. 42 (3), pp. 183-96. - Publication Year :
- 1992
-
Abstract
- Cytosol (C) (100,000 x g/60 min, supernatant) from liver, brain and testis (Wistar male rats) are shown to contain insulin degrading activity (C-IDA). The regulation of C-IDA in these fractions by ligands that activate G protein and PKC were examined C-IDA from liver, brain and testis was inhibited 76%; 64% and 50% by 50 mM F- respectively. Chromatography of C fraction from liver on Sephadex G-50 in presence of 1 M (NH4)2SO4 and 20% (v/v) glycerol (experimental condition to remove guanine nucleotides from G proteins) decreased in about 3-fold aluminum fluoride effect on C-IDA. Mg++ (from 5mM to 10 mM) enhanced fluoride effects by inhibiting fully C-IDA. Phosphatidylserine in presence of ATP completely inhibited C-IDA; this inhibition was 31.3% mediated by a phosphorylation reaction. It is concluded that cytosol from different tissues contain proteins capable to associate ligands as aluminum fluoride and PS to regulate C-IDA. It is proposed a mechanism of protein-protein interaction to modulate C-IDA.
- Subjects :
- Adenosine Triphosphate pharmacology
Ammonium Sulfate pharmacology
Animals
Brain drug effects
Brain metabolism
Cytosol drug effects
Depression, Chemical
GTP-Binding Proteins metabolism
In Vitro Techniques
Iodine Radioisotopes
Liver drug effects
Liver metabolism
Magnesium pharmacology
Male
Protein Kinase C metabolism
Rats
Rats, Wistar
Swine
Testis drug effects
Testis metabolism
Cytosol metabolism
Fluorides pharmacology
Insulin metabolism
Phosphatidylserines pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0327-6309
- Volume :
- 42
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Acta physiologica, pharmacologica et therapeutica latinoamericana : organo de la Asociacion Latinoamericana de Ciencias Fisiologicas y [de] la Asociacion Latinoamericana de Farmacologia
- Publication Type :
- Academic Journal
- Accession number :
- 1343587