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Typing of human group A rotavirus with alkaline phosphatase-labeled oligonucleotide probes.
- Source :
-
Journal of medical virology [J Med Virol] 1992 Jul; Vol. 37 (3), pp. 192-6. - Publication Year :
- 1992
-
Abstract
- Rotavirus (RV) in stools of children less than 1 year of age with diarrhea in Bangkok in 1989 were serotyped by monoclonal enzyme immunoassay (MEIA). RNA extracted from these specimens was tested for hybridization with alkaline phosphatase (AP) and 32P-labeled oligonucleotides constructed from the nucleotide sequences of VP7 of human G types 1 (HuG1Ac), 2 (HuG2Ac), 3 (HuG3Ac), and 4 (HuG4Ac). Of 148 specimens that contained RV, 72% (106/148) hybridized with RV G type specific AP-labeled oligonucleotides compared to 47% (70/148) that were serotyped by MEIA (P less than 0.001). Of 68 specimens that contained only one VP7 serotype (G-type), as identified by MEIA, 94% (16/17) of G1, 90% (27/30) of G2, 57% (4/7) of G3, and 36% (5/14) of G4 RV hybridized with the AP-labeled HuG1Ac, HuG2Ac, HuG3Ac, and HuG4Ac oligonucleotides, respectively. The probes for G1, 2, 3, and 4 RV were specific for each G type. The results of hybridizing specimens with 32P- and AP-labeled oligonucleotides were similar. After transcription and amplification of cDNA of gene 9, AP-labeled RV G type specific oligonucleotides hybridized with 90% (134/148) of RV specimens. The high sensitivity of these nonimmunological techniques could be of value in identifying G types of RV during vaccine trials.
- Subjects :
- Alkaline Phosphatase
Humans
Infant
Molecular Probe Techniques statistics & numerical data
Rotavirus immunology
Rotavirus Infections microbiology
Rotavirus Infections prevention & control
Sensitivity and Specificity
Serotyping methods
Thailand
Viral Vaccines isolation & purification
Oligonucleotide Probes
Rotavirus classification
Rotavirus genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0146-6615
- Volume :
- 37
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Journal of medical virology
- Publication Type :
- Academic Journal
- Accession number :
- 1331307
- Full Text :
- https://doi.org/10.1002/jmv.1890370308