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Cloning of the mouse hypothalamic preprothyrotropin-releasing hormone (TRH) cDNA and tissue distribution of its mRNA.
- Source :
-
Brain research. Molecular brain research [Brain Res Mol Brain Res] 1992 Jun; Vol. 14 (1-2), pp. 131-5. - Publication Year :
- 1992
-
Abstract
- A complementary DNA (cDNA) for mouse hypothalamic preprothyrotropin-releasing hormone (TRH) was isolated and characterized using three different combinations of the polymerase chain reaction (PCR). Using this cDNA, we examined the tissue distribution of expression of the mouse preproTRH gene and evaluated the evolutionary basis of the preproTRH gene by comparison of the cloned sequence with sequences of preproTRH in other species. The deduced protein sequence of the mouse preproTRH contained 256 amino acids featured by possessing an insertion of one amino acid as compared with that of rat preproTRH. Five repetitive copies of the TRH progenitor sequence (Lys-Arg-Gln-His-Pro-Gly-Arg/Lys-Arg) were found in the mouse preproTRH. Northern blot analysis showed an apparent single band of hypothalamic mRNA of approximately 1600 base pairs in length. The homology of the coding region of the mouse preproTRH with the rat and human preproTRH is 92 and 65% at the nucleic acid level, respectively, and 88 and 56% at the amino acid level, respectively. By means of the PCR procedure, a characteristic expression of the preproTRH mRNA was observed solely in the mouse hypothalamus and testis. Moreover, it was noteworthy to find that the degree of homology to the region containing the sixth TRH-coding sequence of the human preproTRH was higher in the mouse preproTRH than the rat counterpart.(ABSTRACT TRUNCATED AT 250 WORDS)
- Subjects :
- Amino Acid Sequence
Animals
Base Sequence
Cloning, Molecular
DNA genetics
Genes
Male
Mice metabolism
Molecular Sequence Data
Open Reading Frames
Peptide Fragments genetics
Polymerase Chain Reaction
RNA, Messenger analysis
Rats genetics
Regulatory Sequences, Nucleic Acid
Repetitive Sequences, Nucleic Acid
Sequence Homology, Nucleic Acid
Species Specificity
Testis metabolism
Hypothalamus metabolism
Mice genetics
Protein Precursors genetics
Thyrotropin-Releasing Hormone genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0169-328X
- Volume :
- 14
- Issue :
- 1-2
- Database :
- MEDLINE
- Journal :
- Brain research. Molecular brain research
- Publication Type :
- Academic Journal
- Accession number :
- 1323011
- Full Text :
- https://doi.org/10.1016/0169-328x(92)90020-c