Back to Search
Start Over
Anoxia-inducible endonuclease activity as a potential basis of the genomic instability of cancer cells.
- Source :
-
Cancer research [Cancer Res] 1992 Aug 15; Vol. 52 (16), pp. 4372-8. - Publication Year :
- 1992
-
Abstract
- Normal rat fibroblasts exhibit a staged response to anoxia which in several respects parallels processes activated in malignant tumor cells. We describe here a new element of the anoxic response, the induction by anoxia of a sequestered endonuclease activity. Such activity is elevated approximately 3-fold within anoxic fibroblasts and during Hirt DNA isolation is able to digest chromatin to produce a nucleosomal ladder. However, DNA is not measurably affected within intact cells, and cells retain complete viability as the endonuclease is induced. The anoxia-inducible endonuclease acts without specificity for DNA sequence. Trace leakage of this endonuclease into the nucleus has obvious potential to underlie the known propensity of anoxic cells to undergo amplification and may be associated with the break-related genomic instability of cancer cells.
- Subjects :
- Animals
Cell Hypoxia physiology
Cell Survival
DNA Probes
DNA, Neoplasm chemistry
DNA, Neoplasm drug effects
Enzyme Induction genetics
Leukemia, Monocytic, Acute enzymology
Leukemia, Monocytic, Acute genetics
Leukemia, Myeloid, Acute enzymology
Leukemia, Myeloid, Acute genetics
Liver Neoplasms, Experimental enzymology
Liver Neoplasms, Experimental genetics
Molecular Weight
Neoplasms enzymology
Plasmids genetics
Rats
Teniposide pharmacology
Tumor Cells, Cultured
Tumor Stem Cell Assay
Cell Hypoxia genetics
DNA, Neoplasm analysis
Endonucleases biosynthesis
Neoplasms genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0008-5472
- Volume :
- 52
- Issue :
- 16
- Database :
- MEDLINE
- Journal :
- Cancer research
- Publication Type :
- Academic Journal
- Accession number :
- 1322786