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Ligand binding kinetics of substance P and neurokinin A receptors stably expressed in Chinese hamster ovary cells and evidence for differential stimulation of inositol 1,4,5-trisphosphate and cyclic AMP second messenger responses.
- Source :
-
Journal of neurochemistry [J Neurochem] 1992 Aug; Vol. 59 (2), pp. 740-5. - Publication Year :
- 1992
-
Abstract
- Stably transfected Chinese hamster ovary cells expressing either the substance P receptor or neurokinin A receptor were constructed, isolated, and characterized. Equilibrium ligand binding studies performed on whole cells demonstrated that cell lines expressing either of these receptors contained a single class of high-affinity binding sites with an apparent KD of 0.16 nM for the substance P receptor and an apparent KD of 2.1 nM for the neurokinin A receptor. The higher affinity of substance P for its receptor was accounted for by both a greater association rate constant and a lesser dissociation rate constant. The time course and extent of ligand-stimulated inositol 1,4,5-trisphosphate mass increases in both cell lines were similar and displayed rapid and transient kinetics. Ligand-stimulated cyclic AMP accumulation was also apparent in the cell lines, although the time course and magnitude of the responses were substantially different, with the neurokinin A receptor mediating a greater and more prolonged response. These studies establish the presence of functional substance P receptors and neurokinin A receptors in the stably transfected cell lines and provide evidence for agonist-dependent differential stimulation of second messenger responses.
- Subjects :
- Animals
Base Sequence
CHO Cells cytology
CHO Cells ultrastructure
Cricetinae
Cyclic AMP physiology
Inositol 1,4,5-Trisphosphate physiology
Kinetics
Ligands
Molecular Sequence Data
Receptors, Neurokinin-1
Receptors, Neurokinin-2
CHO Cells metabolism
Cyclic AMP metabolism
Inositol 1,4,5-Trisphosphate metabolism
Receptors, Neurotransmitter metabolism
Second Messenger Systems physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0022-3042
- Volume :
- 59
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Journal of neurochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 1321234
- Full Text :
- https://doi.org/10.1111/j.1471-4159.1992.tb09430.x