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Reduced levels of transforming growth factor-beta type I receptor in human gastric carcinomas.
- Source :
-
Japanese journal of cancer research : Gann [Jpn J Cancer Res] 1992 Jan; Vol. 83 (1), pp. 86-92. - Publication Year :
- 1992
-
Abstract
- The expressions of transforming growth factor beta (TGF-beta) and its receptor and TGF-beta inhibitory element (TIE)-binding protein were examined on human gastric carcinomas by Northern blot hybridization, immunohistochemistry, affinity labeling and gel retardation analysis. TGF-beta mRNA was expressed in tumor and normal tissues at various levels. Immunohistochemically, TGF-beta expression was confirmed to be present within tumor cells. Out of the 17 human gastric carcinoma tissues, 14 (82%) showed a reduction in the level of type I receptor (65 kDa) for TGF-beta when compared to corresponding normal mucosas. Interestingly, in seven of the 14 tumors the level of TIE-binding protein in the tumor tissue was lower than that in normal mucosa. Human gastric carcinoma cell line TMK-1, whose growth was inhibited by TGF-beta, had only type I receptor for TGF-beta and showed a high level of TIE-binding protein. Conversely, MKN-1, a TGF-beta-resistant cell line, exhibited an extremely low level of TGF-beta receptor and had no TIE-binding protein. These results overall indicate that although human gastric carcinoma cells produced TGF-beta, they showed a reduction in TGF-beta type I receptor and a low level of TIE-binding protein, resulting in escape from growth inhibition by TGF-beta.
- Subjects :
- Aged
Carrier Proteins metabolism
Cell Line metabolism
DNA Replication
Female
Humans
Latent TGF-beta Binding Proteins
Male
Middle Aged
Receptors, Transforming Growth Factor beta
Intracellular Signaling Peptides and Proteins
Receptors, Cell Surface analysis
Stomach Neoplasms metabolism
Transforming Growth Factor beta analysis
Subjects
Details
- Language :
- English
- ISSN :
- 0910-5050
- Volume :
- 83
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Japanese journal of cancer research : Gann
- Publication Type :
- Academic Journal
- Accession number :
- 1312080
- Full Text :
- https://doi.org/10.1111/j.1349-7006.1992.tb02356.x