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Construction of a genetically defined Salmonella typhi Ty2 aroA, aroC mutant for the engineering of a candidate oral typhoid-tetanus vaccine.
- Source :
-
Vaccine [Vaccine] 1992; Vol. 10 (1), pp. 53-60. - Publication Year :
- 1992
-
Abstract
- The construction of a Salmonella typhi Ty2 strain harbouring defined deletions in both the aroA and aroC genes is described. These deletions have been fully defined at the molecular level by DNA sequencing and have been introduced in such a way that no foreign DNA remains in the S. typhi genome. This strain is attenuated in mice when given by the intraperitoneal route suspended in hog gastric mucin and is attenuated to a similar level to strains harbouring deletions in aroA or aroC alone indicating that both lesions are capable of attenuating independently. We have used this defined S. typhi aroA aroC strain to express stably a non-toxic 50 kDa fragment of tetanus toxin (fragment C) from a gene incorporated into the chromosome. This strain has the advantage of harbouring no antibiotic-resistance markers and we consider it to be a candidate bivalent oral typhoid-tetanus vaccine.
- Subjects :
- Administration, Oral
Amino Acid Sequence
Animals
Chromosome Deletion
Chromosome Mapping
Cloning, Molecular
DNA Transposable Elements genetics
DNA, Bacterial genetics
Escherichia coli genetics
Gene Expression genetics
Mice
Mice, Inbred BALB C
Molecular Sequence Data
Mutation genetics
Peptide Fragments genetics
Salmonella typhi immunology
Tetanus Toxin genetics
Salmonella typhi genetics
Tetanus Toxoid genetics
Typhoid-Paratyphoid Vaccines genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0264-410X
- Volume :
- 10
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Vaccine
- Publication Type :
- Academic Journal
- Accession number :
- 1311488
- Full Text :
- https://doi.org/10.1016/0264-410x(92)90420-o