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A tissue-specific knockout reveals that Gata1 is not essential for Sertoli cell function in the mouse.
- Source :
-
Nucleic acids research [Nucleic Acids Res] 2003 Sep 15; Vol. 31 (18), pp. 5405-12. - Publication Year :
- 2003
-
Abstract
- The transcription factor Gata1 is essential for the development of erythroid cells. Consequently, Gata1 null mutants die in utero due to severe anaemia. Outside the haematopoietic system, Gata1 is only expressed in the Sertoli cells of the testis. To elucidate the function of Gata1 in the testis, we made a Sertoli cell-specific knockout of the Gata1 gene in the mouse. We deleted a normally functioning 'floxed' Gata1 gene in pre-Sertoli cells in vivo through the expression of Cre from a transgene driven by the Desert Hedgehog promoter. Surprisingly, Gata1 null testes developed to be morphologically normal, spermatogenesis was not obviously affected and expression levels of putative Gata1 target genes, and other Gata factors, were not altered. We conclude that expression of Gata1 in Sertoli cells is not essential for testis development or spermatogenesis in the mouse.
- Subjects :
- Animals
Binding Sites genetics
Cells, Cultured
DNA-Binding Proteins genetics
Erythroid-Specific DNA-Binding Factors
Female
Fertility genetics
GATA1 Transcription Factor
Gene Expression
Gene Expression Regulation, Developmental
Integrases genetics
Integrases metabolism
Male
Mice
Mice, Inbred C57BL
Mice, Knockout
Mice, Transgenic
RNA, Messenger genetics
RNA, Messenger metabolism
Recombination, Genetic
Reverse Transcriptase Polymerase Chain Reaction
Sertoli Cells cytology
Testis embryology
Testis metabolism
Transcription Factors genetics
Viral Proteins genetics
Viral Proteins metabolism
DNA-Binding Proteins metabolism
Sertoli Cells metabolism
Transcription Factors metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1362-4962
- Volume :
- 31
- Issue :
- 18
- Database :
- MEDLINE
- Journal :
- Nucleic acids research
- Publication Type :
- Academic Journal
- Accession number :
- 12954777
- Full Text :
- https://doi.org/10.1093/nar/gkg723