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The Arabidopsis NPR1 disease resistance protein is a novel cofactor that confers redox regulation of DNA binding activity to the basic domain/leucine zipper transcription factor TGA1.
- Source :
-
The Plant cell [Plant Cell] 2003 Sep; Vol. 15 (9), pp. 2181-91. - Publication Year :
- 2003
-
Abstract
- The Arabidopsis NPR1 protein is essential for regulating salicylic acid-dependent gene expression during systemic acquired resistance. NPR1 interacts differentially with members of the TGA class of basic domain/Leu zipper transcription factors and regulates their DNA binding activity. Here, we report that although TGA1 does not interact with NPR1 in yeast two-hybrid assays, treatment with salicylic acid induces the interaction between these proteins in Arabidopsis leaves. This phenomenon is correlated with a reduction of TGA1 Cys residues. Furthermore, site-directed mutagenesis of TGA1 Cys-260 and Cys-266 enables the interaction with NPR1 in yeast and Arabidopsis. Together, these results indicate that TGA1 relies on the oxidation state of Cys residues to mediate the interaction with NPR1. An intramolecular disulfide bridge in TGA1 precludes interaction with NPR1, and NPR1 can only stimulate the DNA binding activity of the reduced form of TGA1. Unlike its animal and yeast counterparts, the DNA binding activity of TGA1 is not redox regulated; however, this property is conferred by interaction with the NPR1 cofactor.
- Subjects :
- Amino Acid Sequence
Arabidopsis metabolism
Arabidopsis microbiology
Arabidopsis Proteins genetics
Bacteria growth & development
Basic-Leucine Zipper Transcription Factors
Cysteine metabolism
DNA-Binding Proteins genetics
Immunity, Innate genetics
Leucine Zippers genetics
Molecular Sequence Data
Mutation
Oxidation-Reduction drug effects
Plant Diseases genetics
Plant Diseases microbiology
Plant Leaves genetics
Plant Leaves metabolism
Plant Leaves microbiology
Plant Proteins genetics
Plant Proteins metabolism
Protein Binding
Saccharomyces cerevisiae genetics
Salicylic Acid pharmacology
Sequence Homology, Amino Acid
Transcription Factors genetics
Transcription Factors metabolism
Two-Hybrid System Techniques
Arabidopsis genetics
Arabidopsis Proteins metabolism
DNA-Binding Proteins metabolism
Nuclear Proteins
Subjects
Details
- Language :
- English
- ISSN :
- 1040-4651
- Volume :
- 15
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- The Plant cell
- Publication Type :
- Academic Journal
- Accession number :
- 12953119
- Full Text :
- https://doi.org/10.1105/tpc.012849