HPLC method with UV detection for evaluation of digoxin tablet dissolution in acidic medium after solid-phase extraction.

Authors :: Jedlicka A
Grafnetterová T
Miller V
Source :: Journal of pharmaceutical and biomedical analysis [J Pharm Biomed Anal] 2003 Sep 15; Vol. 33 (1), pp. 109-15.
Publication Year :: 2003
Abstract: A simple and reliable method for the evaluation of dissolution of digoxin tablets in 0.01 M hydrochloric acid was developed. Digoxin and its degradation products after solid-phase extraction using C18 Sep-Pak cartridges were evaluated. Analyses were performed on C18 column (LiChrospher RP-18e, 5 microm, 125 x 4.0 mm), as mobile phase water and acetonitrile (72:28, v/v) were used. Detection wavelength was 218 nm. Identity of digoxin degradation products was confirmed by HPLC-MS.

Subjects :: Algorithms
Chromatography, High Pressure Liquid
Hydrogen-Ion Concentration
Indicators and Reagents
Reference Standards
Reproducibility of Results
Solutions
Spectrophotometry, Ultraviolet
Tablets
Cardiotonic Agents analysis
Digoxin analysis

Full Text Access

Tools