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Validation of colorimetric assay to detect complement-mediated antibody-dependent bactericidal activity against serogroups B and C Neisseria meningitidis.
- Source :
-
Biologicals : journal of the International Association of Biological Standardization [Biologicals] 2003 Sep; Vol. 31 (3), pp. 209-12. - Publication Year :
- 2003
-
Abstract
- Colorimetric serum bactericidal assay (cSBA), based on the addition of glucose and a pH indicator to the culture medium after the bactericidal reaction, was validated. The precision measured as repeatability, intermediated precision, and reproducibility was determined as a percentage in titer coincidence between replicas >/=50. Moreover the use of the freeze-dried complement was evaluated in comparison to the traditionally stored by freezing. The results were the following: precision: titer +/-1 two-fold dilution (except for the highly positive serum against serogroup B, where there was titer +/-2 dilutions) percentage in titer coincidence: >/=50. The known titer or +/-1 two-fold dilution was found in the sera titrated with the complement either frozen or freeze-dried. Concluding, cSBA showed to be highly precise, allowing also the use of freeze-dried complement which is another important advantage for this kind of assay.
- Subjects :
- Animals
Complement Hemolytic Activity Assay
Humans
Rabbits
Reproducibility of Results
Antibodies, Bacterial immunology
Blood Bactericidal Activity
Complement System Proteins immunology
Meningococcal Infections immunology
Neisseria meningitidis, Serogroup B immunology
Neisseria meningitidis, Serogroup C immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1045-1056
- Volume :
- 31
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Biologicals : journal of the International Association of Biological Standardization
- Publication Type :
- Academic Journal
- Accession number :
- 12935810
- Full Text :
- https://doi.org/10.1016/s1045-1056(03)00060-5