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Fission yeast Rhp51 is required for the maintenance of telomere structure in the absence of the Ku heterodimer.
- Source :
-
Nucleic acids research [Nucleic Acids Res] 2003 Sep 01; Vol. 31 (17), pp. 5054-63. - Publication Year :
- 2003
-
Abstract
- The Schizosaccharomyces pombe Ku70-Ku80 heterodimer is required for telomere length regulation. Lack of pku70+ results in telomere shortening and striking rearrangements of telomere-associated sequences. We found that the rearrangements of telomere-associated sequences in pku80+ mutants are Rhp51 dependent, but not Rad50 dependent. Rhp51 bound to telomere ends when the Ku heterodimer was not present at telomere ends. We also found that the single-stranded G-rich tails increased in S phase in wild-type strains, while deletion of pku70+ increased the single-stranded overhang in both G2 and S phase. Based on these observations, we propose that Rhp51 binds to the G-rich overhang and promotes homologous pairing between two different telomere ends in the absence of Ku heterodimer. Moreover, pku80 rhp51 double mutants showed a significantly reduced telomere hybridization signal. Our results suggest that, although Ku heterodimer sequesters Rhp51 from telomere ends to inhibit homologous recombination activity, Rhp51 plays important roles for the maintenance of telomere ends in the absence of the Ku heterodimer.
- Subjects :
- Antigens, Nuclear chemistry
Antigens, Nuclear metabolism
DNA, Fungal genetics
DNA, Fungal metabolism
DNA-Binding Proteins chemistry
Dimerization
Fungal Proteins genetics
Fungal Proteins metabolism
G2 Phase
Ku Autoantigen
Meiosis genetics
Mutation
Poly G genetics
Poly G metabolism
Protein Binding
Rad51 Recombinase
S Phase
Schizosaccharomyces genetics
Schizosaccharomyces physiology
Schizosaccharomyces pombe Proteins genetics
Spores, Fungal genetics
Spores, Fungal growth & development
Telomere metabolism
Antigens, Nuclear genetics
DNA Helicases
DNA-Binding Proteins genetics
DNA-Binding Proteins metabolism
Schizosaccharomyces pombe Proteins metabolism
Telomere genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1362-4962
- Volume :
- 31
- Issue :
- 17
- Database :
- MEDLINE
- Journal :
- Nucleic acids research
- Publication Type :
- Academic Journal
- Accession number :
- 12930956
- Full Text :
- https://doi.org/10.1093/nar/gkg718