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Low levels of endogenous oxidative damage cluster levels in unirradiated viral and human DNAs.
- Source :
-
Free radical biology & medicine [Free Radic Biol Med] 2003 Sep 01; Vol. 35 (5), pp. 495-503. - Publication Year :
- 2003
-
Abstract
- Ionizing radiation induces bistranded DNA damage clusters-two or more oxidized bases, abasic, sites or strand breaks on opposing strands within a few helical turns-but it is not known if clusters are also formed in unirradiated DNA in solution or in unirradiated cultured human cells. The frequencies of endogenous oxidized purine clusters (recognized by Escherichia coli Fpg protein), oxidized pyrimidine clusters (recognized by Nth protein), and abasic clusters (cleavage by Nfo protein) were determined using quantitative gel electrophoresis, electronic imaging, and number average length analysis. Methods of DNA isolation and storage were found to affect cluster levels significantly. In bacteriophage T7 DNA prepared using stringent conditions, the frequencies of these clusters were <1/Mbp. In DNA from unirradiated human 28SC monocytes, the levels of such clusters were, at most, a few per gigabase pair.
- Subjects :
- Bacteriophage T7 genetics
DNA-Formamidopyrimidine Glycosylase metabolism
Deoxyribonuclease (Pyrimidine Dimer) metabolism
Deoxyribonuclease IV (Phage T4-Induced) metabolism
Electrophoresis, Agar Gel
Escherichia coli virology
Escherichia coli Proteins metabolism
Gamma Rays
Humans
Monocytes metabolism
Monocytes radiation effects
Oxidation-Reduction
Apurinic Acid radiation effects
DNA radiation effects
DNA Damage
DNA, Viral radiation effects
Oxidative Stress radiation effects
Purines radiation effects
Pyrimidines radiation effects
Subjects
Details
- Language :
- English
- ISSN :
- 0891-5849
- Volume :
- 35
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Free radical biology & medicine
- Publication Type :
- Academic Journal
- Accession number :
- 12927599
- Full Text :
- https://doi.org/10.1016/s0891-5849(03)00327-7