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[Synthesis, cloning, expression and antigenicity of the therapeutic multi-epitope gene of hepatitis B virus and of its products].
- Source :
-
Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA [Di Yi Jun Yi Da Xue Xue Bao] 2003 Aug; Vol. 23 (8), pp. 802-5. - Publication Year :
- 2003
-
Abstract
- Objective: To study the synthesis, cloning, expression and antigenicity of therapeutic multi-epitope gene of hepatitis B virus.<br />Methods: The therapeutic multi-epitope gene of hepatitis B virus was synthesized and cloned into the vector pWR450-1, then was expressed in E.coli and the products were purified. The immunogenicity of the expressed protein was analyzed by Western-blotting.<br />Results: Recombinant plasmid PWR/BPT was constructed successfully and the protein of multi-epitope gene of hepatitis B virus was expressed in E. coli, which showed ideal antigenicity by Western-blotting.<br />Conclusions: The designed multi-epitope therapeutic gene of hepatitis B virus was proved to be correct and the expressed protein may be a good therapeutic vaccine.
Details
- Language :
- Chinese
- ISSN :
- 1000-2588
- Volume :
- 23
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA
- Publication Type :
- Academic Journal
- Accession number :
- 12919903