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A simplified method for purification of annexin V from human placenta.
- Source :
-
Preparative biochemistry & biotechnology [Prep Biochem Biotechnol] 2003 Aug; Vol. 33 (3), pp. 209-15. - Publication Year :
- 2003
-
Abstract
- A simplified procedure for purification of annexin V from human placenta was developed. At first, the protein was separated from other proteins in membrane bound form in the presence of Ca2+, then was extracted with EDTA and purified by affinity chromatography on PAAG-immobilized phosphatidylserine. The purified protein gave a single band with a molecular weight of 35,000 in SDS-PAGE.
Details
- Language :
- English
- ISSN :
- 1082-6068
- Volume :
- 33
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Preparative biochemistry & biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 12916812
- Full Text :
- https://doi.org/10.1081/PB-120022989