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OTX1 compensates for OTX2 requirement in regionalisation of anterior neuroectoderm.
OTX1 compensates for OTX2 requirement in regionalisation of anterior neuroectoderm.
- Source :
-
Gene expression patterns : GEP [Gene Expr Patterns] 2003 Aug; Vol. 3 (4), pp. 497-501. - Publication Year :
- 2003
-
Abstract
- Otx genes play a relevant role in specification, maintenance and patterning of anterior neuroectoderm. OTX1 and OTX2 proteins share extensive codogenic similarity even though in OTX1 these regions of homology are separated by stretches of amino acid insertions. From 1 to 3 somites stage onwards, Otx1 and Otx2 are largely coexpressed, but only Otx2 is expressed during gastrulation. To determine whether OTX1 and OTX2 gene products share common biochemical properties, mouse models replacing Otx1 with Otx2 and vice versa have been generated. These studies have indicated a remarkable functional equivalence between the two proteins. Nevertheless, it was still debated whether OTX1 is functionally equivalent to OTX2 in early anterior neuroectoderm. To address this issue we generated a new mouse model (hOtx1(2FL)) replacing only the coding sequence and introns of Otx2 with the human Otx1 codogenic sequence. hOtx1(2FL/2FL) and hOtx1(2FL/-) mice were viable, fertile and exhibited an apparently normal behaviour. hOtx1 mRNA was correctly transcribed under the Otx2 transcriptional control and, similarly, the hOTX1 protein was properly distributed and quantitatively very similar if not identical to that of OTX2. Patterning and regionalisation of forebrain and midbrain were unaffected as revealed by the expression of diagnostic genes which are highly sensitive to reduction of OTX proteins, such as Fgf8, Pax2 and Gbx2.
- Subjects :
- Animals
Body Patterning
DNA-Binding Proteins genetics
DNA-Binding Proteins metabolism
Female
Fibroblast Growth Factor 8
Fibroblast Growth Factors genetics
Fibroblast Growth Factors metabolism
Gene Expression Regulation, Developmental
Homeodomain Proteins genetics
Homeodomain Proteins metabolism
Humans
In Situ Hybridization
Male
Mice
Mice, Inbred C57BL
Mice, Knockout
Nerve Tissue Proteins genetics
Otx Transcription Factors
PAX2 Transcription Factor
Phenotype
RNA, Messenger
Trans-Activators genetics
Transcription Factors genetics
Transcription Factors metabolism
Ectoderm physiology
Homeodomain Proteins physiology
Mesencephalon embryology
Nerve Tissue Proteins physiology
Prosencephalon embryology
Trans-Activators physiology
Transcription Factors physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1567-133X
- Volume :
- 3
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Gene expression patterns : GEP
- Publication Type :
- Academic Journal
- Accession number :
- 12915318
- Full Text :
- https://doi.org/10.1016/s1567-133x(03)00056-5