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17beta-estradiol upregulates the expression of peroxisome proliferator-activated receptor alpha and lipid oxidative genes in skeletal muscle.
- Source :
-
Journal of molecular endocrinology [J Mol Endocrinol] 2003 Aug; Vol. 31 (1), pp. 37-45. - Publication Year :
- 2003
-
Abstract
- This study examined the actions of 17beta-estradiol (E(2)) and progesterone on the regulation of the peroxisome proliferator-activated receptors (PPARalpha and PPARgamma) family of nuclear transcription factors and the mRNA abundance of key enzymes involved in fat oxidation, in skeletal muscle. Specifically, carnitine palmitoyltransferase I (CPT I), beta-3-hydroxyacyl CoA dehydrogenase (beta-HAD), and pyruvate dehydrogenase kinase 4 (PDK4) were examined. Sprague-Dawley rats were ovariectomized and treated with placebo (Ovx), E(2), progesterone, or both hormones in combination (E+P). Additionally, sham-operated rats were treated with placebo (Sham) to serve as controls. Hormone (or vehicle only) delivery was via time release pellets inserted at the time of surgery, 15 days prior to analysis. E(2) treatment increased PPARalpha mRNA expression and protein content (P<0.05), compared with Ovx treatment. E(2) also resulted in upregulated mRNA of CPT I and PDK4 (P<0.05). PPARgamma mRNA expression was also increased (P<0.05) by E(2) treatment, although protein content remained unaltered. These data demonstrate the novel regulation of E(2) on PPARalpha and genes encoding key proteins that are pivotal in regulating skeletal muscle lipid oxidative flux.
- Subjects :
- Animals
Base Sequence
Body Weight
DNA Primers
Estradiol physiology
Fatty Acids, Nonesterified blood
Female
Lipids genetics
Muscle, Skeletal drug effects
Ovariectomy
Oxidation-Reduction
Progesterone physiology
Protein Isoforms genetics
RNA, Messenger genetics
RNA, Messenger metabolism
Rats
Rats, Sprague-Dawley
Receptors, Cytoplasmic and Nuclear drug effects
Transcription Factors drug effects
Estradiol pharmacology
Gene Expression Regulation drug effects
Lipid Metabolism
Muscle, Skeletal physiology
Progesterone pharmacology
Receptors, Cytoplasmic and Nuclear genetics
Transcription Factors genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0952-5041
- Volume :
- 31
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of molecular endocrinology
- Publication Type :
- Academic Journal
- Accession number :
- 12914523
- Full Text :
- https://doi.org/10.1677/jme.0.0310037