Identification of important bases for the self-cleavage activity at two single-stranded regions of genomic HDV ribozyme.

Authors :: Nishikawa S
Suh YA
Kumar PK
Kawakami J
Nishikawa F
Taira K
Source :: Nucleic acids symposium series [Nucleic Acids Symp Ser] 1992 (27), pp. 41-2.
Publication Year :: 1992
Abstract: In order to determine important bases at two single-stranded regions [SSrA (726-731 nt) and SSrB (762-766)] derived mainly from secondary structure models in genomic hepatitis delta virus (HDV) ribozyme possessing self-cleavage activity, we have constructed several point mutants at these two regions on the HDV88 molecule (683-770). Among the bases at SSrA and SSrB regions C763 was found to play an essential role during self-cleavage process since substitutions to any other bases viz. A or G or U completely abolished the activity.

Subjects :: Base Sequence
Molecular Sequence Data
Nucleic Acid Conformation
Point Mutation
RNA, Catalytic genetics
RNA, Viral genetics
Hepatitis Delta Virus genetics
RNA, Catalytic metabolism
RNA, Viral metabolism

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