Back to Search
Start Over
Control of cellulose synthase complex localization in developing xylem.
- Source :
-
The Plant cell [Plant Cell] 2003 Aug; Vol. 15 (8), pp. 1740-8. - Publication Year :
- 2003
-
Abstract
- Cellulose synthesis in the developing xylem vessels of Arabidopsis requires three members of the cellulose synthase (CesA) gene family. In young vessels, these three proteins localize within the cell, whereas in older vessels, all three CesA proteins colocalize with bands of cortical microtubules that mark the sites of secondary cell wall deposition. In the absence of one subunit, however, the remaining two subunits are retained in the cell, demonstrating that all three CesA proteins are required to assemble a functional complex. CesA proteins with altered catalytic activity localize normally, suggesting that cellulose synthase activity is not required for this localization. Cortical microtubule arrays are required continually to maintain normal CesA protein localization. By contrast, actin microfilaments do not colocalize with the CesA proteins and are unlikely to play a direct role in their localization. Green fluorescent protein-tagged CesA reveals a novel process in which the structure and/or local environment of the cellulose synthase complex is altered rapidly.
- Subjects :
- Arabidopsis genetics
Arabidopsis Proteins genetics
Base Sequence
Cytoskeleton enzymology
DNA, Recombinant genetics
Fluorescent Antibody Technique
Genes, Plant
Glucosyltransferases genetics
Green Fluorescent Proteins
Luminescent Proteins genetics
Luminescent Proteins metabolism
Multigene Family
Mutation
Organelles enzymology
Recombinant Fusion Proteins genetics
Recombinant Fusion Proteins metabolism
Arabidopsis enzymology
Arabidopsis growth & development
Arabidopsis Proteins metabolism
Glucosyltransferases metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1040-4651
- Volume :
- 15
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- The Plant cell
- Publication Type :
- Academic Journal
- Accession number :
- 12897249
- Full Text :
- https://doi.org/10.1105/tpc.012815