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High-throughput gene expression profiling indicates dysregulation of intestinal cell cycle mediators and growth factors during primary simian immunodeficiency virus infection.
- Source :
-
Virology [Virology] 2003 Jul 20; Vol. 312 (1), pp. 84-94. - Publication Year :
- 2003
-
Abstract
- During primary simian immunodeficiency virus (SIV) infection, CD4+ T cells are severely depleted in gut-associated lymphoid tissue (GALT), while CD8+ T-cell numbers dramatically increase. To gain an understanding of the molecular basis of this disruption in T-cell homeostasis, host gene expression was monitored in longitudinal jejunum tissue biopsies from SIV-infected rhesus macaques by DNA microarray analysis. Transcription of cyclin E1, CDC2, retinoblastoma, transforming growth factor (TGF), fibroblast growth factor (FGF), and interleukin-2 was repressed while cyclins B1 and D2 and transcription factor E2F were upregulated, indicating a complex dysregulation of growth and proliferation within the intestinal mucosa. Innate, cell-mediated, and humoral immune responses were markedly upregulated in animals that significantly reduced their viral loads and retained more intestinal CD4+ T cells. We conclude that the alterations in intestinal gene expression during primary SIV infection were characteristic of a broad-range immune response, and reflective of the efficacy of viral suppression.
- Subjects :
- Animals
Antigen Presentation
Cluster Analysis
Gene Expression Regulation
Intestinal Mucosa immunology
Macaca mulatta
Male
RNA analysis
RNA genetics
Simian Acquired Immunodeficiency Syndrome immunology
Stress, Physiological genetics
T-Lymphocytes immunology
Transcription, Genetic
Viremia genetics
Cell Cycle genetics
Gene Expression Profiling
Growth Substances genetics
Intestinal Mucosa metabolism
Intestinal Mucosa virology
Simian Acquired Immunodeficiency Syndrome genetics
Simian Immunodeficiency Virus physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0042-6822
- Volume :
- 312
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Virology
- Publication Type :
- Academic Journal
- Accession number :
- 12890623
- Full Text :
- https://doi.org/10.1016/s0042-6822(03)00207-1