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In vitro anti-inflammatory activity of Phlebodium decumanum. Modulation of tumor necrosis factor and soluble TNF receptors.
- Source :
-
International immunopharmacology [Int Immunopharmacol] 2003 Sep; Vol. 3 (9), pp. 1293-9. - Publication Year :
- 2003
-
Abstract
- The immunomodulatory activity of a standardized water soluble fraction of the fern Phlebodium decumanum (EXPLY-37) previously shown to have "in vivo" anti-inflammatory activity was analyzed "in vitro". This extract inhibited tumor necrosis factor (TNF) production by macrophages activated with lipopolysaccharide (LPS) or LPS plus interferon (IFN)-gamma. In contrast, nitric oxide (NO) and interleukin (IL)-1beta production were not affected in the same cultures, whereas IL-6 production was partially inhibited. More interestingly, EXPLY-37 increased the release of soluble TNF-receptor 2 (sTNFR2) and of IL-1R antagonist (IL-1Ra) but not of sTNFR1, by activated macrophages. EXPLY-37 had no effect on T lymphocyte activation, measured as proliferation as well as expression of early and late cell surface antigens CD69, CD25 (IL-2R-alpha) and CD71 (transferrin receptor) at the cell membrane. At the molecular level, EXPLY-37 did not inhibit the activation of the nuclear factor kappa B (NF-kappaB) transcription factor by TNF. In summary, EXPLY-37 has two anti-inflammatory activities "in vitro": it decreases TNF production and increases IL-1Ra and sTNFR2, which may be able to neutralize IL-1 and TNF activity, respectively.
- Subjects :
- Adjuvants, Immunologic isolation & purification
Adult
Animals
Anti-Inflammatory Agents, Non-Steroidal isolation & purification
Antigens, CD genetics
Cell Line drug effects
Cell Line metabolism
Drug Evaluation, Preclinical
HeLa Cells drug effects
HeLa Cells metabolism
Humans
Interleukin 1 Receptor Antagonist Protein
Leukocytes, Mononuclear drug effects
Leukocytes, Mononuclear metabolism
Lipopolysaccharides pharmacology
Lymphocyte Activation drug effects
Macrophages drug effects
Macrophages metabolism
Mice
NF-kappa B metabolism
Plant Extracts isolation & purification
Receptors, Tumor Necrosis Factor genetics
Receptors, Tumor Necrosis Factor, Type II
Sialoglycoproteins genetics
T-Lymphocytes drug effects
Transcription, Genetic drug effects
Adjuvants, Immunologic pharmacology
Anti-Inflammatory Agents, Non-Steroidal pharmacology
Antigens, CD biosynthesis
Plant Extracts pharmacology
Plants, Medicinal chemistry
Polypodiaceae chemistry
Receptors, Tumor Necrosis Factor biosynthesis
Sialoglycoproteins biosynthesis
Tumor Necrosis Factor-alpha metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1567-5769
- Volume :
- 3
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- International immunopharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 12890427
- Full Text :
- https://doi.org/10.1016/S1567-5769(03)00117-6