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Glycosaminoglycans affect the interaction of human plasma kallikrein with plasminogen, factor XII and inhibitors.
- Source :
-
Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas [Braz J Med Biol Res] 2003 Aug; Vol. 36 (8), pp. 1055-9. Date of Electronic Publication: 2003 Jul 23. - Publication Year :
- 2003
-
Abstract
- Human plasma kallikrein, a serine proteinase, plays a key role in intrinsic blood clotting, in the kallikrein-kinin system, and in fibrinolysis. The proteolytic enzymes involved in these processes are usually controlled by specific inhibitors and may be influenced by several factors including glycosaminoglycans, as recently demonstrated by our group. The aim of the present study was to investigate the effect of glycosaminoglycans (30 to 250 micro/ml) on kallikrein activity on plasminogen and factor XII and on the inhibition of kallikrein by the plasma proteins C1-inhibitor and antithrombin. Almost all available glycosaminoglycans (heparin, heparan sulfate, bovine and tuna dermatan sulfate, chondroitin 4- and 6-sulfates) reduced (1.2 to 3.0 times) the catalytic efficiency of kallikrein (in a nanomolar range) on the hydrolysis of plasminogen (0.3 to 1.8 microM) and increased (1.9 to 7.7 times) the enzyme efficiency in factor XII (0.1 to 10 microM) activation. On the other hand, heparin, heparan sulfate, and bovine and tuna dermatan sulfate improved (1.2 to 3.4 times) kallikrein inhibition by antithrombin (1.4 microM), while chondroitin 4- and 6-sulfates reduced it (1.3 times). Heparin and heparan sulfate increased (1.4 times) the enzyme inhibition by the C1-inhibitor (150 nM).
- Subjects :
- Animals
Cattle
Complement C1 Inactivator Proteins drug effects
Complement C1 Inhibitor Protein
Cysteine Proteinase Inhibitors pharmacology
Factor XII physiology
Humans
Plasma Kallikrein antagonists & inhibitors
Plasma Kallikrein physiology
Factor XII drug effects
Fibrinolytic Agents pharmacology
Glycosaminoglycans pharmacology
Plasma Kallikrein drug effects
Plasminogen drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 0100-879X
- Volume :
- 36
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas
- Publication Type :
- Academic Journal
- Accession number :
- 12886459
- Full Text :
- https://doi.org/10.1590/s0100-879x2003000800011