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Comparative proteomic analysis of proliferating and functionally differentiated mammary epithelial cells.
- Source :
-
Molecular & cellular proteomics : MCP [Mol Cell Proteomics] 2003 Oct; Vol. 2 (10), pp. 1039-54. Date of Electronic Publication: 2003 Jul 28. - Publication Year :
- 2003
-
Abstract
- Proliferation and differentiation of mammary epithelial cells are governed by hormonal stimuli, cell-cell, and cell-matrix interactions. Terminal differentiation of mammary epithelial cells depends upon the action of the lactogenic hormones, insulin, glucocorticoids, and prolactin that enable them to synthesize and secrete milk proteins. These differentiated cells are polarized and carry out vectorial transport of milk constituents across the apical plasma membrane. To gain additional insights into the mechanisms governing differentiation of mammary epithelial cells, we identified proteins whose expression distinguishes proliferating from differentiated mammary epithelial cells. For this purpose we made use of the HC11 mammary epithelial line, which is capable of differentiation in response to lactogenic hormones. Using two-dimensional gel electrophoresis and mass spectrometry, we found about 60 proteins whose expression levels changed in between these two differentiation states. Bioinformatic analysis revealed differential expression of cytoskeletal components, molecular chaperones and regulators of protein folding and stability, calcium-binding proteins, and components of RNA-processing pathways. The actin cytoskeleton is asymmetrically distributed in differentiated epithelial cells, and the identification of proteins involved in mRNA binding and localization suggests that asymmetry might in part be achieved by controlling cellular localization of mRNAs. The proteins identified provide insights into the differentiation of mammary epithelial cells and the regulation of this process.
- Subjects :
- Animals
Base Sequence
Calcium-Binding Proteins biosynthesis
Calcium-Binding Proteins genetics
Cell Differentiation drug effects
Cell Line
Cell Proliferation drug effects
DNA genetics
Dexamethasone pharmacology
Electrophoresis, Gel, Two-Dimensional
Epithelial Cells cytology
Epithelial Cells drug effects
Epithelial Cells metabolism
Extracellular Matrix metabolism
Female
Glycolysis
Insulin pharmacology
Intracellular Signaling Peptides and Proteins genetics
Intracellular Signaling Peptides and Proteins metabolism
Mammary Glands, Animal drug effects
Mice
Prolactin pharmacology
Proteasome Endopeptidase Complex biosynthesis
Proteasome Endopeptidase Complex genetics
Protein Biosynthesis
Proteins genetics
Proteomics
RNA Processing, Post-Transcriptional
RNA, Messenger genetics
RNA, Messenger metabolism
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Transcription Factors biosynthesis
Transcription Factors genetics
Mammary Glands, Animal cytology
Mammary Glands, Animal metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1535-9476
- Volume :
- 2
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Molecular & cellular proteomics : MCP
- Publication Type :
- Academic Journal
- Accession number :
- 12885951
- Full Text :
- https://doi.org/10.1074/mcp.M300032-MCP200