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Somatic embryogenesis in loblolly pine (Pinus taeda L.): improving culture initiation with abscisic acid and silver nitrate.
- Source :
-
Plant cell reports [Plant Cell Rep] 2003 Sep; Vol. 22 (2), pp. 85-95. Date of Electronic Publication: 2003 Jul 19. - Publication Year :
- 2003
-
Abstract
- Loblolly pine ( Pinus taeda L.) culture initiation was improved by the addition of abscisic acid (ABA) (3.7 micro M), silver nitrate (20 micro M), and guanosine 3',5'-cyclic monophosphate, 8-bromo-, sodium salt (10 micro M) to the medium and by raising cytokinin levels in the presence of 50 mg/l activated carbon (AC). Basal medium contained modified 1/2-P6 salts, 50 mg/l AC, Cu and Zn added to compensate for adsorption by AC, 1.5% maltose, 2% myo-inositol, 500 mg/l casamino acids, 450 mg/l glutamine, 2 mg/l alpha-naphthaleneacetic acid (NAA), 0.55 mg/l 6-benzylaminopurine (BA), 0.53 mg/l kinetin, and 2 g/l Gelrite. Across 32 open-pollinated families initiation ranged from 0 to 53.4%, with an average of 17.9%. Further optimization of cytokinins to 0.63 mg/l BA and 0.61 mg/l kinetin along with the removal of ABA maintained initiation at 18.2% across 19 families. Survival of 2001 new initiations was tracked for 4-6 months. Survival averaged 28.8%. A test of 68 new initiations tracked closely for 4 months demonstrated that at least 80% of the cultures lost did not grow after transfer to the multiplication media, suggesting that many new initiations abort during the initiation process.
- Subjects :
- Carbon pharmacology
Culture Media pharmacology
Culture Techniques
Cyclic GMP pharmacology
Cytokinins pharmacology
Germination drug effects
Pinus taeda drug effects
Pinus taeda growth & development
Seeds drug effects
Seeds growth & development
Abscisic Acid pharmacology
Cyclic GMP analogs & derivatives
Pinus taeda embryology
Seeds embryology
Silver Nitrate pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0721-7714
- Volume :
- 22
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Plant cell reports
- Publication Type :
- Academic Journal
- Accession number :
- 12879261
- Full Text :
- https://doi.org/10.1007/s00299-003-0673-y