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Detection of circovirus with a polymerase chain reaction in the ostrich (Struthio camelus) on a farm in The Netherlands.
- Source :
-
Veterinary microbiology [Vet Microbiol] 2003 Aug 29; Vol. 95 (1-2), pp. 27-38. - Publication Year :
- 2003
-
Abstract
- This study describes for the first time the presence of circoviruses in ostrich tissue including embryos. A polymerase chain reaction (PCR) was used for the detection of the virus in liver samples. The use of a polymerase for low copy detection significantly increased the sensitivity of the test as well as a Southern blot. Viral DNA could be detected in chicks and eggs that did not hatch. For localisation of the virus in the liver in situ hybridisation was performed on a selection of positive liver tissues.
- Subjects :
- Animals
Base Sequence
Blotting, Southern veterinary
Circoviridae Infections virology
Circovirus genetics
DNA, Viral chemistry
DNA, Viral genetics
In Situ Hybridization veterinary
Liver virology
Molecular Sequence Data
Netherlands
Polymerase Chain Reaction veterinary
Bird Diseases virology
Circoviridae Infections veterinary
Circovirus isolation & purification
Struthioniformes
Subjects
Details
- Language :
- English
- ISSN :
- 0378-1135
- Volume :
- 95
- Issue :
- 1-2
- Database :
- MEDLINE
- Journal :
- Veterinary microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 12860074
- Full Text :
- https://doi.org/10.1016/s0378-1135(03)00122-6