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Disturbing GTP-binding protein function through microinjection into the visual cell of Limulus.
- Source :
-
Zeitschrift fur Naturforschung. C, Journal of biosciences [Z Naturforsch C J Biosci] 1992 Nov-Dec; Vol. 47 (11-12), pp. 915-21. - Publication Year :
- 1992
-
Abstract
- We have tested the action of three agents microinjected into the ventral nerve photoreceptor of Limulus on the electrical response to dim light. 1. A monoclonal antibody (mAb 4A) against the G alpha subunit of frog transducin reduces the size of the receptor current to 60%, suggesting an interaction with G alpha in the Limulus photoreceptor. 2. Injection of Clostridium botulinum ADP-ribosyltransferase C3 reduces the size to 46%; latency is not affected. The results imply that small GTP-binding proteins play a functional role in photoreception of invertebrates. 3. Injection of GDP-beta-S reduces dose-dependently the size of the receptor current to 15% and prolongs the latency to 200%, presumably by reducing number and rate of G-protein activations.
Details
- Language :
- English
- ISSN :
- 0939-5075
- Volume :
- 47
- Issue :
- 11-12
- Database :
- MEDLINE
- Journal :
- Zeitschrift fur Naturforschung. C, Journal of biosciences
- Publication Type :
- Academic Journal
- Accession number :
- 1285831
- Full Text :
- https://doi.org/10.1515/znc-1992-11-1220