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Epitope mapping of the Brucella melitensis BP26 immunogenic protein: usefulness for diagnosis of sheep brucellosis.
- Source :
-
Clinical and diagnostic laboratory immunology [Clin Diagn Lab Immunol] 2003 Jul; Vol. 10 (4), pp. 647-51. - Publication Year :
- 2003
-
Abstract
- Sequencing of bp26, the gene encoding the Brucella sp. immunogenic BP26 periplasmic protein, was performed in the reference strains of Brucella abortus, B. suis, and B. ovis. The three bp26 sequences were almost identical to that published for B. melitensis 16M bp26, and only minor nucleotide substitutions, without modifying the amino acid sequence, were observed between species. The bp26 genes of the seven B. abortus biovar reference strains and B. abortus S19 and RB51 vaccine strains were also sequenced. Again, only minor differences were found. Surprisingly, the bp26 nucleotide sequence for B. abortus S19 was almost identical to that found for B. melitensis 16M and differed from the sequence described previously by others (O. L. Rossetti, A. I. Arese, M. L. Boschiroli, and S. L. Cravero, J. Clin. Microbiol. 34:165-169, 1996) for the same B. abortus strain. The epitope mapping of BP26, performed by using a panel of monoclonal antibodies and recombinant DNA techniques, allowed the identification of an immunodominant region of the protein interesting for the diagnosis of B. melitensis and B. ovis infection in sheep. A recombinant fusion protein containing this region of BP26 reacted indeed, in Western blotting, as the entire recombinant BP26 against sera from B. melitensis- or B. ovis-infected sheep while it avoided false-positive reactions observed with sera from Brucella-free sheep when using the entire recombinant BP26. Thus, use of this recombinant fusion protein instead the entire recombinant BP26 could improve the specific serological diagnosis of B. melitensis or B. ovis infection in sheep.
- Subjects :
- Amino Acid Sequence
Animals
Antigens, Bacterial genetics
Bacterial Proteins genetics
Brucella melitensis genetics
Brucella ovis immunology
Brucella suis immunology
Brucellosis diagnosis
Brucellosis microbiology
Genes, Bacterial
Immunodominant Epitopes genetics
Membrane Proteins genetics
Molecular Sequence Data
Recombinant Fusion Proteins immunology
Sequence Alignment
Sequence Homology, Amino Acid
Sheep
Sheep Diseases microbiology
Species Specificity
Antigens, Bacterial immunology
Bacterial Proteins immunology
Brucella melitensis immunology
Brucellosis veterinary
Epitope Mapping
Immunodominant Epitopes immunology
Membrane Proteins immunology
Sheep Diseases diagnosis
Subjects
Details
- Language :
- English
- ISSN :
- 1071-412X
- Volume :
- 10
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Clinical and diagnostic laboratory immunology
- Publication Type :
- Academic Journal
- Accession number :
- 12853399
- Full Text :
- https://doi.org/10.1128/cdli.10.4.647-651.2003