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Cloning and characterization of a novel splicing isoform of USF1.
- Source :
-
International journal of molecular medicine [Int J Mol Med] 2003 Aug; Vol. 12 (2), pp. 161-7. - Publication Year :
- 2003
-
Abstract
- The ubiquitous basic helix-loop-helix transcription factor USFs encoded by two distinct genes (USF1 and USF2) recognize a core motif, CACGTG, termed E box and regulate the expression of a variety of genes. USF1 and USF2 proteins form homo- and heterodimers to bind the target core motif DNA. Here, we report the molecular cloning and functional characterization of a novel alternative splicing variant of human USF1 (hUSF1), termed USF1/BD. Compared with USF1 wild-type (wt), USF1/BD lacks the N-terminal transactivation domain. Cloning and characterization of the hUSF1 genomic region revealed that USF1/BD is generated by excising the sequence corresponding to a part of exon 4. In transiently transfected cells, USF1/BD was localized in the nucleus and repressed the promoter activity of the human angiotensinogen gene. In vitro translated USF1/BD possessed DNA binding activity as a homodimer and a heterodimer with USF1 (wt). These results suggest that USF1/BD plays a role as a modulator of USF1 to control the expression of target genes.
- Subjects :
- Angiotensinogen genetics
Animals
Base Sequence
Binding Sites
Cells, Cultured
Cloning, Molecular
Codon, Terminator
Dimerization
E-Box Elements
Exons
Gene Expression Regulation
Helix-Loop-Helix Motifs
Humans
Mice
Molecular Sequence Data
Promoter Regions, Genetic
Protein Structure, Tertiary
Sequence Homology, Nucleic Acid
Transcription Factors metabolism
Upstream Stimulatory Factors
Alternative Splicing
DNA-Binding Proteins
Transcription Factors genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1107-3756
- Volume :
- 12
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- International journal of molecular medicine
- Publication Type :
- Academic Journal
- Accession number :
- 12851711