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Structural characterization of a novel branching pattern in the lipopolysaccharide from nontypeable Haemophilus influenzae.
- Source :
-
European journal of biochemistry [Eur J Biochem] 2003 Jul; Vol. 270 (14), pp. 2979-91. - Publication Year :
- 2003
-
Abstract
- Structural analysis of the lipopolysaccharide (LPS) from nontypeable Haemophilus influenzae strain 981 has been achieved using NMR spectroscopy and ESI-MS on O-deacylated LPS and core oligosaccharide (OS) material as well as by ESI-MSn on permethylated dephosphorylated OS. A heterogeneous glycoform population was identified, resulting from the variable length of the OS branches attached to the glucose residue in the common structural element of H. influenzae LPS, l-alpha-d-Hepp-(1-->2)-[PEtn-->6]-l-alpha-d-Hepp-(1-->3)-[beta-d-Glcxp-(1-->4)]-l-alpha-d-Hepp-(1-->5)-[PPEtn-->4]-alpha-Kdop-(2-->6)-Lipid A. Notably, the O-6 position of the beta-d-Glcp residue was either substituted by PCho or the disaccharide branch beta-d-Galp-(1-->4)-d-alpha-d-Hepp, while the O-4 position was substituted by the globotetraose unit, beta-d-GalpNAc-(1-->3)-alpha-d-Galp-(1-->4)-beta-d-Galp-(1-->4)-beta-d-Glcp, or sequentially truncated versions thereof. This is the first time a branching sugar residue has been reported in the outer-core region of H. influenzae LPS. Additionally, a PEtn group was identified at O-3 of the distal heptose residue in the inner-core.
- Subjects :
- Acetylation
Carbohydrate Conformation
Carbohydrate Sequence
Glycosylation
Haemophilus influenzae genetics
Haemophilus influenzae metabolism
Heptoses analysis
Hexoses analysis
Humans
Lipopolysaccharides metabolism
Molecular Sequence Data
Nuclear Magnetic Resonance, Biomolecular
Otitis Media microbiology
Phosphorylation
Phosphorylcholine
Spectrometry, Mass, Electrospray Ionization methods
Haemophilus influenzae chemistry
Lipopolysaccharides chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 0014-2956
- Volume :
- 270
- Issue :
- 14
- Database :
- MEDLINE
- Journal :
- European journal of biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 12846831
- Full Text :
- https://doi.org/10.1046/j.1432-1033.2003.03675.x