Back to Search
Start Over
Angiogenic effects of injected VEGF165 and sVEGFR-1 (sFLT-1) in a rat flap model.
- Source :
-
The Journal of surgical research [J Surg Res] 2003 May 01; Vol. 111 (1), pp. 136-42. - Publication Year :
- 2003
-
Abstract
- Background: Injections of single-dose vascular endothelial growth factor (VEGF)(165) have been advocated as a therapeutic tool for angiogenesis in ischemic flaps. We challenged this thesis by employing both VEGF(165) and vascular endothelial growth factor receptor-1 (VEGFR-1) (for competitive inhibition of VEGF signal transduction) in different experimental settings of an ischemic rat flap model.<br />Material and Methods: 80 isogenic rats were divided in two groups of 40 animals (groups 1A-1D and 2A-2D). The ischemic target was a 7 x 7-cm epigastric island flap, based on the right inferior epigastric pedicle. Group 1 received flap treatment 1 week prior to flap elevation by test substance injection into its flap panniculus carnosus: 1 ml NaCl 0.9% (1A), 1 ml Dulbecco's modified Eagle's medium (1B), 1.0 microg VEGF(165) (1C), and 10 microg sFLT-1 with 1.0 microg VEGF(165) (1D). sFLT-1 is a soluble receptor for VEGF and is able to prevent VEGF signaling through the cell surface receptor. Group 2 had the same flap treatment at the day of flap elevation.<br />Results: In group 1C we found the most vital flap tissue, without reaching significance. Compared with group 1D, however, significantly more flap tissue maintained vital. In groups 2A-2D, no significant results were found with respect to flap survival.<br />Conclusions: Local application of single-dose VEGF(165) 1 week prior to ischemia dose not have significant clinical angiogenic effects. In this experimental setting, VEGF(165)-induced angiogenic effects can be significantly inhibited by adding sFLT1 in vivo. A single-dose of VEGF(165) under ischemic conditions causes no significantly better flap survival in this model.
- Subjects :
- Animals
DNA biosynthesis
Endothelial Growth Factors metabolism
Endothelium, Vascular metabolism
Female
Humans
Injections
Intercellular Signaling Peptides and Proteins metabolism
Ischemic Preconditioning
Lymphokines metabolism
Models, Animal
Rats
Rats, Inbred Lew
Solubility
Vascular Endothelial Growth Factor A
Vascular Endothelial Growth Factor Receptor-1 metabolism
Vascular Endothelial Growth Factors
Endothelial Growth Factors administration & dosage
Intercellular Signaling Peptides and Proteins administration & dosage
Lymphokines administration & dosage
Neovascularization, Physiologic drug effects
Surgical Flaps
Vascular Endothelial Growth Factor Receptor-1 administration & dosage
Subjects
Details
- Language :
- English
- ISSN :
- 0022-4804
- Volume :
- 111
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- The Journal of surgical research
- Publication Type :
- Academic Journal
- Accession number :
- 12842458
- Full Text :
- https://doi.org/10.1016/s0022-4804(03)00084-2