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Gene structure, expression and identification of a new CTP:phosphocholine cytidylyltransferase beta isoform.
- Source :
-
Biochimica et biophysica acta [Biochim Biophys Acta] 2003 Jul 04; Vol. 1633 (1), pp. 1-12. - Publication Year :
- 2003
-
Abstract
- CTP:phosphocholine cytidylyltransferase (CCT) is a key regulatory enzyme in phosphatidylcholine (PtdCho) biosynthesis, and in mammals, there are two distinct genes that encode enzymes that catalyze this reaction. This work defines the structures of both the murine CCT genes (Pcyt1a and Pcyt1b) and identifies a new CCT protein, CCTbeta3, with a unique amino terminus that arises from an alternate initiation exon. CCTalpha is expressed in all tissues, and is most abundant in liver, kidney and heart. A second CCTalpha transcript is described that initiates from a separate untranslated exon that is most highly expressed in testis. The CCTbeta isoforms are most highly expressed in brain and reproductive tissues. CCTbeta3 is not expressed in embryonic brain tissues, but is a significant transcript in the adult. These data suggest unique roles for the CCT protein isoforms in the differential regulation of PtdCho biosynthesis in specific tissues.
- Subjects :
- Alternative Splicing
Amino Acid Sequence
Animals
Brain enzymology
CHO Cells
Cells, Cultured
Choline-Phosphate Cytidylyltransferase chemistry
Cricetinae
Exons
Genetic Vectors
Liver enzymology
Mice
Molecular Sequence Data
Phosphatidylcholines metabolism
Protein Isoforms chemistry
Protein Structure, Tertiary
RNA, Messenger genetics
RNA, Messenger metabolism
Substrate Specificity
Tissue Distribution
Transcription, Genetic
Choline-Phosphate Cytidylyltransferase genetics
Choline-Phosphate Cytidylyltransferase metabolism
Protein Isoforms genetics
Protein Isoforms metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0006-3002
- Volume :
- 1633
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Biochimica et biophysica acta
- Publication Type :
- Academic Journal
- Accession number :
- 12842190
- Full Text :
- https://doi.org/10.1016/s1388-1981(03)00067-2