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Transfection of fluorescent probed antisense compounds: L-cysteine derivatives of nucleic acid bases.
- Source :
-
Nucleic acids research. Supplement (2001) [Nucleic Acids Res Suppl] 2001 (1), pp. 135-6. - Publication Year :
- 2001
-
Abstract
- Antisense with L-cysteine derivative (CAS) can recognize DNA and forms the complementary duplex with DNA. So the properties of CAS in vitro and in vivo were examined in this study. CAS was resistant to proteinase K and stabilized RNA against RNase HI. Moreover using fluorescent CAS, the localization was observed by fluorescence microscope and confocal microscope. As a result, CASs were accumulated inside the nucleus in NG108-15.
- Subjects :
- Adenine analogs & derivatives
Animals
Cell Nucleus chemistry
Endopeptidase K metabolism
Fluorescein
Fluorescent Dyes
Microscopy, Fluorescence
Nucleic Acids chemistry
Oligodeoxyribonucleotides, Antisense analysis
Oligodeoxyribonucleotides, Antisense genetics
Oligodeoxyribonucleotides, Antisense metabolism
Peptides chemistry
Ribonuclease H metabolism
Thymine analogs & derivatives
Transfection
Tumor Cells, Cultured
Carbocysteine chemistry
Oligodeoxyribonucleotides, Antisense chemistry
Subjects
Details
- Language :
- English
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Nucleic acids research. Supplement (2001)
- Publication Type :
- Academic Journal
- Accession number :
- 12836301
- Full Text :
- https://doi.org/10.1093/nass/1.1.135