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Alternate-strand triple-helix formation by the 3'-3'-linked oligodeoxynucleotides with the intercalators at the junction point.
- Source :
-
Nucleic acids research. Supplement (2001) [Nucleic Acids Res Suppl] 2001 (1), pp. 11-2. - Publication Year :
- 2001
-
Abstract
- 3'-3'-Linked oligodeoxynucleotides (ODNs) with the anthraquinonyl group at the junction point were synthesized on a DNA synthesizer using a controlled pore glass (CPG), which has pentaerythritol carrying the intercalator at one of the four hydroxymethyl groups. Stability of the triplexes with the target duplexes was studied by thermal denaturation. The 3'-3'-linked ODNs with the anthraquinonyl group enhanced the thermal stability of the triplexes when compared with those without the intercalator and the unmodified nonamer. The inhibitory activity of the 3'-3'-linked ODNs against the cleavage of the target DNA by the restriction enzyme Hind III was tested. It was found that the 3'-3'-linked ODN with the anthraquinonyl group at the junction point inhibited the cleavage by the enzyme more effectively than the nonamer and the 3'-3'-linked ODN without the intercalator.
- Subjects :
- Base Sequence
DNA chemistry
DNA pharmacology
Deoxyribonuclease HindIII metabolism
Enzyme Inhibitors chemical synthesis
Enzyme Inhibitors chemistry
Enzyme Inhibitors pharmacology
Nucleic Acid Denaturation
Oligodeoxyribonucleotides chemistry
Oligodeoxyribonucleotides pharmacology
Temperature
Anthraquinones chemistry
DNA chemical synthesis
Intercalating Agents chemistry
Oligodeoxyribonucleotides chemical synthesis
Subjects
Details
- Language :
- English
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Nucleic acids research. Supplement (2001)
- Publication Type :
- Academic Journal
- Accession number :
- 12836239
- Full Text :
- https://doi.org/10.1093/nass/1.1.11