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[Effects of rotating clinostat simulated weightlessness on the differentiation-related gene expression of ROS17/2.8 cells].
- Source :
-
Hang tian yi xue yu yi xue gong cheng = Space medicine & medical engineering [Space Med Med Eng (Beijing)] 2003 Apr; Vol. 16 (2), pp. 120-2. - Publication Year :
- 2003
-
Abstract
- Objective: To investigate the effects of rotating clinostat simulated weightlessness on the differentiation-related gene expression of ROS17/2.8 (rat osteosarcoma) cells.<br />Method: Cultured ROS17/2.8 cells were exposed to rotating clinostat simulated weightlessness for 24, 48 and 72 h with control group in 1 G condition. Total RNA in cells was isolated and reverse transcription PCR analysis was made. Gene expression of type I collagen alpha 1 chain and alkaline phosphatase were examined by their ratios to beta-actin.<br />Result: The expression of type I collagen alpha I chain and alkaline phosphatase (ALP) gene was significantly lower after 72 h exposure to clinostat simulated weightlessness than that of the control group (P<0.01).<br />Conclusion: Differentiation of rat osteosarcoma cells was reduced after exposure to rotating clinostat simulated weightlessness for 72 h.
Details
- Language :
- Chinese
- ISSN :
- 1002-0837
- Volume :
- 16
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Hang tian yi xue yu yi xue gong cheng = Space medicine & medical engineering
- Publication Type :
- Academic Journal
- Accession number :
- 12830838