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High-resolution visualization of oxygen distribution in the liver in vivo.
- Source :
-
American journal of physiology. Gastrointestinal and liver physiology [Am J Physiol Gastrointest Liver Physiol] 2004 Jan; Vol. 286 (1), pp. G37-44. Date of Electronic Publication: 2003 Jun 26. - Publication Year :
- 2004
-
Abstract
- Microcirculatory failure after stress events results in mismatch in oxygen supply and demand. Determination of tissue oxygen distribution in vivo may help elucidate mechanisms of injury, but present methods have limited resolution. Male Sprague-Dawley rats were anesthetized, prepared for intravital microscopy, and received intravenously the oxygen-sensitive fluorescent dye Tris(1,10-phenanthroline)ruthenium(II) chloride hydrate [Ru(phen)3(2+)]. An impaired hepatic oxygen distribution was induced by either phenylephrine or hemorrhage. Intensity of Ru(phen)3(2+) fluorescence was compared with NADH autofluorescence indicating changes in the mitochondrial redox potential. Ethanol was injected to affect the NADH-to-NAD+ ratio without altering the P(O2). Infusion of Ru(phen)3(2+) resulted in a heterogeneous fluorescence under baseline conditions reflecting the physiological acinar P(O2) distribution. A decrease in oxygen supply due to phenylephrine or hemorrhage was paralleled by an increase in Ru(phen)3(2+) and NADH fluorescence reflecting an impaired mitochondrial redox state. Ethanol did not alter Ru(phen)3(2+) fluorescence but increased NADH fluorescence indicating independence of P(O2) and redox state imaging. Intravenous administration of Ru(phen)3(2+) for intravital videomicroscopy represents a new method to visualize the hepatic tissue P(O2). Combined with NADH autofluorescence, it provides additional information regarding the tissue redox state.
- Subjects :
- Animals
Aspartate Aminotransferases metabolism
Chemical and Drug Induced Liver Injury metabolism
Chemical and Drug Induced Liver Injury pathology
Fluorescence
Fluorescent Dyes toxicity
Hemodynamics drug effects
Hemodynamics physiology
Hemorrhage enzymology
Hemorrhage metabolism
L-Lactate Dehydrogenase metabolism
Liver Circulation physiology
Male
Microcirculation physiology
Microscopy, Video
NAD metabolism
Oxidation-Reduction
Rats
Rats, Sprague-Dawley
Liver anatomy & histology
Liver metabolism
Oxygen Consumption physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0193-1857
- Volume :
- 286
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- American journal of physiology. Gastrointestinal and liver physiology
- Publication Type :
- Academic Journal
- Accession number :
- 12829437
- Full Text :
- https://doi.org/10.1152/ajpgi.00041.2003