Effect of different laboratory techniques for guanidinium-phenol-chloroform RNA extraction on A260/A280 and on accuracy of mRNA quantitation by reverse transcriptase-PCR.

Subjects

Subjects :

Base Sequence
Cells, Cultured
Chloroform
Endothelium, Vascular cytology
Guanidine
Guanidines
Humans
Molecular Sequence Data
Phenol
Phenols
Sensitivity and Specificity
Solvents
DNA analysis
Polymerase Chain Reaction
RNA, Messenger isolation & purification
RNA-Directed DNA Polymerase
Spectrophotometry, Ultraviolet

Details

Language :

English

ISSN :

1054-9803

Volume :

1

Issue :

4

Database :

MEDLINE

Journal :

PCR methods and applications

Publication Type :

Academic Journal

Accession number :

1282433

Full Text :

https://doi.org/10.1101/gr.1.4.286