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Dysregulated expression of IFN-gamma and IL-10 and impaired IFN-gamma-mediated responses at different disease stages in patients with genital herpes simplex virus-2 infection.
- Source :
-
Clinical and experimental immunology [Clin Exp Immunol] 2003 Jul; Vol. 133 (1), pp. 97-107. - Publication Year :
- 2003
-
Abstract
- Cell-mediated T-helper type-1 (Th1) responses play a vital role in the immunopathogenesis of genital infections caused by herpes simplex virus 2 (HSV-2). We investigated the role of Th responses in HSV-2 infection at different disease stages by analysing the production of Th cytokines in HSV-stimulated peripheral blood mononuclear cells (PBMCs). IFN-gamma production decreased over time following a recurrence, whereas levels of IL-10, and to a lesser extent IL-2, remained elevated during this period. In addition, PBMCs from asymptomatic seropositive individuals produced high levels of IFN-gamma and low levels of IL-10, in contrast to individuals with a history of genital ulcers. Following a recurrence, virus copy number in the genital lesions decreased progressively over time, in a manner similar to IFN-gamma production by HSV-2-stimulated PBMCs. Enhanced production of IFN-gamma may modulate HSV replication and B7 expression on monocytic cells of HSV-infected individuals. In contrast to seronegative controls, IFN-gamma failed to enhance B7 expression on monocytic cells of HSV-infected individuals. In addition, monocytic cells from HSV-2-infected individuals with recurrent disease supported greater HSV replication than did those of HSV-infected asymptomatic individuals or seronegative controls. Furthermore, addition of IFN-gamma resulted in enhanced HSV replication in monocytic cells of HSV-infected individuals with recurrent disease, in contrast to the inhibition observed in HSV-seropositive asymptomatic individuals and seronegative controls. Taken together, our results suggest that dysregulated production of IFN-gamma at different disease stages and the impaired ability of monocytic cells to respond to IFN-gamma may play a role in the pathogenesis of recurrent genital herpes disease.
- Subjects :
- Case-Control Studies
Cells, Cultured
DNA, Viral analysis
Enzyme-Linked Immunosorbent Assay methods
Flow Cytometry
Herpes Genitalis virology
Humans
Interleukin-2 analysis
Interleukin-4 analysis
Leukocytes, Mononuclear immunology
Polymerase Chain Reaction methods
Recurrence
Virus Activation
Virus Replication
Herpes Genitalis immunology
Herpesvirus 2, Human physiology
Interferon-gamma analysis
Interleukin-10 analysis
Th1 Cells immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0009-9104
- Volume :
- 133
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Clinical and experimental immunology
- Publication Type :
- Academic Journal
- Accession number :
- 12823283
- Full Text :
- https://doi.org/10.1046/j.1365-2249.2003.02183.x